4XIF
Human OGT in complex with UDP-5S-GlcNAc and substrate peptide (keratin-7)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.873 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 275.138, 275.138, 143.136 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 3.200 |
R-factor | 0.21593 |
Rwork | 0.216 |
R-free | 0.23893 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.356 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | REFMAC |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 3.200 |
Number of reflections | 105151 |
<I/σ(I)> | 1.7 |
Completeness [%] | 99.0 |
Redundancy | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 1.45 M potassium phosphate, 10 mM EDTA, 1% (w/v) xylitol |