4XIB
Crystal structure of the MZM-REP domains of Mind bomb 1 in complex with fly Delta N-box peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-08 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 74.131, 74.131, 170.174 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.049 - 2.147 |
R-factor | 0.2117 |
Rwork | 0.209 |
R-free | 0.24440 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.780 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.190 |
High resolution limit [Å] | 2.147 | 2.147 |
Rmerge | 0.993 | |
Number of reflections | 24766 | |
<I/σ(I)> | 17.6 | 2.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 293.15 | Protein at 6 mg/mL in 25 mM HEPES 7.4, 500 mM NaCl, 10% w/v glucose, 100 mM Arg, 2 mM TCEP Precipitant: 1.5 M (NH4)2SO4, 20% v/v glycerol, HEPES pH 7.6 1:1 drop volume |