4XIA

STRUCTURES OF D-XYLOSE ISOMERASE FROM ARTHROBACTER STRAIN B3728 CONTAINING THE INHIBITORS XYLITOL AND D-SORBITOL AT 2.5 ANGSTROMS AND 2.3 ANGSTROMS RESOLUTION, RESPECTIVELY

Experimental procedure

Spacegroup name	P 31 2 1
Unit cell lengths	105.800, 105.800, 153.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 * - 2.300
R-factor	0.156 *
RMSD bond length	0.020
RMSD bond angle	0.046
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.062 *
Total number of observations	127622 *
Number of reflections	44186 *
Completeness [%]	98.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *		Akins, J., (1986) Biochim.Biophys.Acta, 874, 375. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	23 (mg/ml)
2	1	drop	ammonium sulfate	1.2 (M)
3	1	reservoir	Tris-acetate	50 (mM)
4	1	reservoir		10 (mM)