4XI6

Crystal structure of the MZM-REP domains of Mind bomb 1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-17
Detector	ADSC QUANTUM 315
Wavelength(s)	1.2562, 1.2822, 1.283
Spacegroup name	P 41 2 2
Unit cell lengths	73.083, 73.083, 170.959
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.939 - 2.040
R-factor	0.2111
Rwork	0.208
R-free	0.24810
RMSD bond length	0.005
RMSD bond angle	0.967
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.080
High resolution limit [Å]	2.040	5.540	2.040
Rmerge	0.067	0.038	0.404
Total number of observations	235966
Number of reflections	29269
<I/σ(I)>	6.5
Completeness [%]	95.2	95.2	53.6
Redundancy	8.1	8.2	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	293.15	1.5 M (NH4)2SO4, 20% v/v glycerol, HEPES pH 7.6