4XI1
Crystal structure of U-box 2 of LubX / LegU2 / Lpp2887 from Legionella pneumophila str. Paris, wild-type
Replaces: 4WZ1Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9790433 |
Spacegroup name | P 4 3 2 |
Unit cell lengths | 160.227, 160.227, 160.227 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.874 - 2.983 |
R-factor | 0.1647 |
Rwork | 0.162 |
R-free | 0.20950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wz2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.052 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.030 |
High resolution limit [Å] | 2.980 | 2.980 |
Rmerge | 0.080 | 0.623 |
Number of reflections | 13171 | |
<I/σ(I)> | 23.58 | 2.7 |
Completeness [%] | 88.7 | 92.7 |
Redundancy | 5.7 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 15 mg/ml protein, V8 protease (1:100), 1.6 M ammonium sulfate, 0.1 M HEPES pH 7.5 and 2% hexanediol |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 15 mg/ml protein, V8 protease (1:100), 1.6 M ammonium sulfate, 0.1 M HEPES pH 7.5 and 2% hexanediol |