4XHY

NADH:FMN oxidoreductase from Paracoccus denitrificans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-18
Detector	MAR CCD 165 mm
Wavelength(s)	0.81
Spacegroup name	P 31 2 1
Unit cell lengths	43.910, 43.910, 154.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	51.550 - 1.530
R-factor	0.16
Rwork	0.158
R-free	0.20500
RMSD bond length	0.019
RMSD bond angle	1.987
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	51.550
High resolution limit [Å]	1.500
Number of reflections	28207
<I/σ(I)>	44.12
Completeness [%]	98.2
Redundancy	8.26

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 4000, ammonium sulfate, ammonium acetate, MES