4XHT
Crystal structure of Timeless_PAB domain native form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.06997 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 66.668, 100.596, 149.554 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.338 - 1.651 |
| R-factor | 0.1861 |
| Rwork | 0.185 |
| R-free | 0.20570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xhw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.090 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.340 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.071 | 0.431 |
| Number of reflections | 59619 | |
| <I/σ(I)> | 29.65 | 4.64 |
| Completeness [%] | 98.4 | 100 |
| Redundancy | 6.3 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | 25% PEG 3350, 200mM sodium acetate |
