4XHR
Structure of a phospholipid trafficking complex, native
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.7 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.968, 74.868, 87.568 |
| Unit cell angles | 90.00, 95.32, 90.00 |
Refinement procedure
| Resolution | 32.080 - 2.550 |
| R-factor | 0.23837 |
| Rwork | 0.236 |
| R-free | 0.27736 |
| Structure solution method | SAD |
| RMSD bond length | 0.150 |
| RMSD bond angle | 1.861 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.080 | 3.000 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.116 | |
| Number of reflections | 17640 | |
| <I/σ(I)> | 11.96 | 11.96 |
| Completeness [%] | 97.2 | 97.17 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 2%(v/v) Tacsimate pH 6.0, 0.1M Bis-Tris pH 6.5, 20%(w/v) PEG3350 |
