4XHN
Bacillus thuringiensis ParM with AMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 55.443, 71.412, 114.094 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.880 - 2.600 |
R-factor | 0.2154 |
Rwork | 0.209 |
R-free | 0.27190 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.814 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.3.0) |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.640 |
High resolution limit [Å] | 2.600 | 6.960 | 2.600 |
Rmerge | 0.039 | 0.012 | 0.377 |
Total number of observations | 99481 | ||
Number of reflections | 14466 | ||
<I/σ(I)> | 19 | ||
Completeness [%] | 99.4 | 99.6 | 90.6 |
Redundancy | 6.9 | 6.5 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 288 | 0.1 M Bis-Tris, pH 6.5, 0.2 M ammonium acetate, 25% PEG 3350 |