4XHN

Bacillus thuringiensis ParM with AMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 2 21 21
Unit cell lengths	55.443, 71.412, 114.094
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.880 - 2.600
R-factor	0.2154
Rwork	0.209
R-free	0.27190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.814
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.640
High resolution limit [Å]	2.600	6.960	2.600
Rmerge	0.039	0.012	0.377
Total number of observations	99481
Number of reflections	14466
<I/σ(I)>	19
Completeness [%]	99.4	99.6	90.6
Redundancy	6.9	6.5	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	288	0.1 M Bis-Tris, pH 6.5, 0.2 M ammonium acetate, 25% PEG 3350