4XHL

Structure of S. cerevisiae Hrr25 1-394 (K38R mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 62 2 2
Unit cell lengths	137.497, 137.497, 94.639
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	119.080 - 3.010
R-factor	0.2679
Rwork	0.267
R-free	0.28470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xh0
RMSD bond length	0.002
RMSD bond angle	0.599
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	119.080	3.170
High resolution limit [Å]	3.010	3.010
Rmerge	0.110	1.252
Number of reflections	10857
<I/σ(I)>	16.8	2
Completeness [%]	99.5	99.4
Redundancy	10.6	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	11	293	0.1 M CAPS pH 11, 0.2 M lithium sulfate, and 1.5-1.6 M ammonium sulfate