4XHH
Structure of C. glabrata Hrr25, Apo state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 72.091, 77.860, 85.078 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.438 - 2.908 |
| R-factor | 0.2043 |
| Rwork | 0.203 |
| R-free | 0.22470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xh0 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.800 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.080 | 3.050 |
| High resolution limit [Å] | 2.890 | 2.890 |
| Rmerge | 0.273 | 0.724 |
| Number of reflections | 11067 | |
| <I/σ(I)> | 10.8 | 2.4 |
| Completeness [%] | 98.9 | 94.4 |
| Redundancy | 5.9 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 10.5 | 293 | 0.1 M CAPS pH 10.5, 0.2 M lithium sulfate, 0.56 M NaH2PO4, 0.66 M K2HPO4 |
