4XHG

Structure of C. glabrata Hrr25 bound to ADP (formate condition)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 21 2 21
Unit cell lengths	72.460, 76.472, 84.187
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.600 - 2.150
R-factor	0.1761
Rwork	0.174
R-free	0.21620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xh0
RMSD bond length	0.007
RMSD bond angle	1.043
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.600	2.270
High resolution limit [Å]	2.150	2.150
Rmerge	0.100	0.491
Number of reflections	25960
<I/σ(I)>	11.4	2.5
Completeness [%]	99.6	99.7
Redundancy	4	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	0.1 M Bis-Tris pH 6.5, 0.2 M sodium formate, 15% PEG 3350, and 5 mM TCEP. Cryoprotected with 20% glycerol.