4XHG
Structure of C. glabrata Hrr25 bound to ADP (formate condition)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 72.460, 76.472, 84.187 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.600 - 2.150 |
R-factor | 0.1761 |
Rwork | 0.174 |
R-free | 0.21620 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xh0 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.043 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.600 | 2.270 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.100 | 0.491 |
Number of reflections | 25960 | |
<I/σ(I)> | 11.4 | 2.5 |
Completeness [%] | 99.6 | 99.7 |
Redundancy | 4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Bis-Tris pH 6.5, 0.2 M sodium formate, 15% PEG 3350, and 5 mM TCEP. Cryoprotected with 20% glycerol. |