4XHA
Crystal structure of Thosea asigna virus RNA-dependent RNA polymerase (RdRP) complexed with Lu3+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-11-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.3404 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 154.050, 224.570, 128.290 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.517 - 3.000 |
R-factor | 0.1852 |
Rwork | 0.182 |
R-free | 0.24390 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.514 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXDE |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.500 | 3.160 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.116 | 0.379 |
Number of reflections | 44236 | |
<I/σ(I)> | 13.3 | 4.5 |
Completeness [%] | 98.7 | 96.5 |
Redundancy | 6.5 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 12% PEG 8K, 750 mM lithium sulfate |