4XHA
Crystal structure of Thosea asigna virus RNA-dependent RNA polymerase (RdRP) complexed with Lu3+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.3404 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 154.050, 224.570, 128.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.517 - 3.000 |
| R-factor | 0.1852 |
| Rwork | 0.182 |
| R-free | 0.24390 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.514 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.500 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.116 | 0.379 |
| Number of reflections | 44236 | |
| <I/σ(I)> | 13.3 | 4.5 |
| Completeness [%] | 98.7 | 96.5 |
| Redundancy | 6.5 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 12% PEG 8K, 750 mM lithium sulfate |
