4XH8
Crystal Structure of E112A/D230A Mutant of Stationary Phase Survival Protein (SurE) from Salmonella typhimurium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97625 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 92.340, 92.340, 150.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.140 - 3.560 |
R-factor | 0.26368 |
Rwork | 0.262 |
R-free | 0.30423 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v4n |
RMSD bond length | 0.005 |
RMSD bond angle | 0.923 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.300 | 3.900 |
High resolution limit [Å] | 3.560 | 3.560 |
Rmerge | 0.560 | |
Number of reflections | 6585 | |
<I/σ(I)> | 4.9 | 1.8 |
Completeness [%] | 81.2 | 82.4 |
Redundancy | 4.3 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.08M Magnesium chloride, 0.1M HEPES (pH 7.5), 15 % polyacrylic acid sodium salt |