4XH3
Mechanistic insights into anchorage of the contractile ring from yeast to humans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2012-05-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.148, 126.116, 54.132 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.669 - 2.100 |
| R-factor | 0.2194 |
| Rwork | 0.218 |
| R-free | 0.24790 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.930 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.000 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 17691 |
| <I/σ(I)> | 14.8 |
| Completeness [%] | 96.1 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 20% (w/v) glycerol, 18% PEG 1500, 100 mM Hepes, 5 mM DTT |
