4XGW
Crystal structure of Escherichia coli Flavin trafficking protein, an FMN transferase, E169K mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 |
Unit cell lengths | 57.216, 70.650, 86.276 |
Unit cell angles | 75.72, 71.91, 69.72 |
Refinement procedure
Resolution | 33.922 - 1.747 |
R-factor | 0.1732 |
Rwork | 0.172 |
R-free | 0.19850 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4xgv |
RMSD bond length | 0.006 |
RMSD bond angle | 0.874 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.780 |
High resolution limit [Å] | 1.747 | 4.750 | 1.750 |
Rmerge | 0.087 | 0.033 | 0.855 |
Total number of observations | 429538 | ||
Number of reflections | 116958 | ||
<I/σ(I)> | 10.3 | 1.8 | |
Completeness [%] | 97.3 | 97.4 | 96 |
Redundancy | 3.7 | 3.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.2 M NaCl, 25% (w/v) PEG 3350, 0.1 M HEPES, 20% (v/v) ethylene glycol; |