4XGV

Crystal structure of Escherichia coli Flavin trafficking protein, an FMN transferase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-03-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	P 1
Unit cell lengths	56.197, 70.645, 85.961
Unit cell angles	75.70, 72.45, 69.28

Refinement procedure

Resolution	31.513 - 1.883
R-factor	0.1645
Rwork	0.163
R-free	0.20080
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2o18
RMSD bond length	0.007
RMSD bond angle	0.956
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.910
High resolution limit [Å]	1.880	5.100	1.880
Rmerge	0.064	0.033	0.581
Total number of observations	190214
Number of reflections	91038
<I/σ(I)>	7.6		1.8
Completeness [%]	96.9	97.7	94.5
Redundancy	2.1	2.1	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.2 M ammonium nitrate, 20% (w/v) PEG 3350, 35% (v/v) ethylene glycol;