4XGT

Structure of RNA Helicase FRH a Critical Component of the Neurospora Crassa Circadian Clock

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	78.841, 113.462, 143.626
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.113 - 3.090
R-factor	0.2351
Rwork	0.232
R-free	0.29530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xgj
RMSD bond length	0.004
RMSD bond angle	0.903
Data scaling software	Aimless (0.1.27)
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1819))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	143.630	143.630	3.260
High resolution limit [Å]	3.090	9.770	3.090
Rmerge	0.124	0.045	1.300
Rpim	0.051	0.019	0.511
Total number of observations	173112	5348	25811
Number of reflections	24298
<I/σ(I)>	12.1	32.9	1.5
Completeness [%]	99.9	99	100
Redundancy	7.1	6.2	7.4
CC(1/2)	0.997	0.996	0.607

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	290	sodium citrate, PEG600