4XGM
Structure of the nuclease subunit of human mitochondrial RNase P (MRPP3) at 1.98A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 43 |
| Unit cell lengths | 99.040, 99.040, 50.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.067 - 1.980 |
| R-factor | 0.194 |
| Rwork | 0.193 |
| R-free | 0.22630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xgl |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.957 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.067 | 2.030 | |
| High resolution limit [Å] | 1.980 | 8.860 | 1.980 |
| Rmerge | 0.093 | 0.021 | 2.025 |
| Rmeas | 0.023 | 2.187 | |
| Number of reflections | 34479 | 421 | 2551 |
| <I/σ(I)> | 16.54 | 69.68 | 1 |
| Completeness [%] | 99.9 | 98.6 | 99.9 |
| Redundancy | 6.8 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | HEPES, PEG 6000, magnesium chloride |
