4XGL
Structure of the nuclease subunit of human mitochondrial RNase P (MRPP3) at 1.8A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 43 |
Unit cell lengths | 99.250, 99.250, 50.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.625 - 1.800 |
R-factor | 0.1899 |
Rwork | 0.189 |
R-free | 0.22760 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.920 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.850 | |
High resolution limit [Å] | 1.800 | 8.050 | 1.800 |
Rmerge | 0.092 | 0.039 | 2.270 |
Rmeas | 0.097 | 0.041 | 2.386 |
Total number of observations | 488725 | ||
Number of reflections | 46217 | 565 | 3393 |
<I/σ(I)> | 12.89 | 49.79 | 0.89 |
Completeness [%] | 99.9 | 99.6 | 99.8 |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | potassium citrate, PEG3350, magnesium chloride |