4XGJ
Crystal structure of a domain of unknown function (DUF1537) from Pectobacterium atrosepticum (ECA3761), Target EFI-511609, APO structure, domain swapped dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-12 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 153.772, 47.844, 63.792 |
Unit cell angles | 90.00, 106.69, 90.00 |
Refinement procedure
Resolution | 41.530 - 1.900 |
R-factor | 0.158 |
Rwork | 0.156 |
R-free | 0.19200 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.295 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.650 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.062 | 0.650 |
Number of reflections | 35312 | |
<I/σ(I)> | 18.9 | 2.9 |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.4 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | Protein (46.8 mg/ml, 10 mM HEPES pH 7.5, 5 mM DTT); Reservoir (MCSG4 (F4); 0.2 M Magnesium Chloride, 0.1 M Tris pH 8.5, 16 %(w/v) PEG 4000); Cryoprotection (80% Reservoir, 20% glycerol) |