4XGA

Crystal structure of BamB and BamA P3-5 complex from E.coli

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-15
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	109.035, 109.642, 49.488
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.357 - 2.150
R-factor	0.1833
Rwork	0.181
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3q54 3q6b
RMSD bond length	0.008
RMSD bond angle	1.160
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.190
High resolution limit [Å]	2.150	2.150
Rmerge	0.043	0.295
Number of reflections	33219
<I/σ(I)>	35.8	5.7
Completeness [%]	99.9	100
Redundancy	6.1	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1% w/v Tryptone, 20% w/v Polyethylene glycol 3350, 0.05M HEPES sodium pH 7.0