4XFX

Structure of the native full-length HIV-1 capsid protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2013-06-28
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 6
Unit cell lengths	92.328, 92.328, 57.340
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.000 - 2.430
R-factor	0.2229
Rwork	0.222
R-free	0.24900
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.805
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.000	80.000	2.470
High resolution limit [Å]	2.430	6.590	2.430
Rmerge	0.077	0.024
Rmeas	0.077	0.025
Rpim	0.026	0.008	0.399
Total number of observations	115799
Number of reflections	10588
<I/σ(I)>	9.2
Completeness [%]	99.7	94.7	100
Redundancy	10.9	9.8	10.1
CC(1/2)		0.999	0.694

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG3350, NaI, MMT