4XFN

Structure of an Amyloid forming peptide AEVVFT from Human Transthyretin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-08-09
Detector	PSI PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	42.750, 9.530, 18.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.117 - 1.850
R-factor	0.158
Rwork	0.154
R-free	0.20080
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.054
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	14.117
High resolution limit [Å]	1.850
Number of reflections	740
<I/σ(I)>	4.71
Completeness [%]	93.7
Redundancy	23.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.4M Ammonium dihydrogen phosphate, 25% Glycerol