4XER
Crystal Structure of C2 form of E112A/H234A Mutant of Stationary Phase Survival Protein (SurE) from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.953720 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.550, 96.520, 94.950 |
| Unit cell angles | 90.00, 98.61, 90.00 |
Refinement procedure
| Resolution | 40.320 - 1.970 |
| R-factor | 0.16763 |
| Rwork | 0.165 |
| R-free | 0.21392 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v4n |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.804 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.320 | 2.070 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.498 | |
| Number of reflections | 104677 | |
| <I/σ(I)> | 142 | 2.6 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 4.6 | 293 | 0.02M Calcium chloride, 0.1M Sodium acetate (pH 4.6), 30 % 2-Methyl 2,4-pentanediol |
