4XEP
Crystal Structure of F222 form of E112A/H234A Mutant of Stationary Phase Survival Protein (SurE) from Salmonella typhimurium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | F 2 2 2 |
| Unit cell lengths | 73.455, 121.653, 143.401 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.500 |
| R-factor | 0.18918 |
| Rwork | 0.187 |
| R-free | 0.22859 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v4n |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.887 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.479 | |
| Number of reflections | 49090 | |
| <I/σ(I)> | 68.1 | 2.4 |
| Completeness [%] | 95.5 | 75.4 |
| Redundancy | 9.7 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 293 | 0.1 M HEPES pH 7.5, 0.02 M MgCl2 hexahydrate, 22% (w/v) Polyacrylic acid 5100 sodium salt |
