4XEN

High pressure protein crystallography of hen egg white lysozyme in complex with Tetra-N-acetylchitotetraose at 920 MPa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	298
Detector technology	CCD
Collection date	2014-12-10
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.75
Spacegroup name	P 43 21 2
Unit cell lengths	76.152, 76.152, 37.962
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.110 - 1.550
R-factor	0.16686
Rwork	0.165
R-free	0.20969
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lzc
RMSD bond length	0.021
RMSD bond angle	2.165
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.580
High resolution limit [Å]	1.550	1.550
Rmerge		0.286
Number of reflections	16785
<I/σ(I)>	44.5	9.59
Completeness [%]	99.3	98.9
Redundancy	7.4	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	Sodium chloride