4XEI

Orthorhombic isomorph of bovine Arp2/3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-15
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	105.574, 156.981, 178.087
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.408 - 3.874
R-factor	0.2815
Rwork	0.279
R-free	0.32850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k8k
RMSD bond length	0.007
RMSD bond angle	1.510
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.874
Rmerge	0.194
Number of reflections	26925
<I/σ(I)>	6.8
Completeness [%]	97.6
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	277	Ethanol 10%, 1,4-butanediol 15%, 100 mM Tris 7.4