4XED
PKD domain of M14-like peptidase from Thermoplasmatales archaeon SCGC AB-540-F20
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 46.186, 48.869, 78.509 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 1.230 |
| R-factor | 0.1394 |
| Rwork | 0.138 |
| R-free | 0.16870 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.435 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.250 |
| High resolution limit [Å] | 1.230 | 1.230 |
| Rmerge | 0.116 | 0.480 |
| Number of reflections | 25859 | |
| <I/σ(I)> | 12.81 | 1.98 |
| Completeness [%] | 98.4 | 89.4 |
| Redundancy | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 0.2 M Ca acetate, 0.1 M Na acetate/acetic acid pH 4.5, 30% PEG 400 |
