4XE2
N-terminal domain of Hsp90 from Dictyostelium discoideum in complex with peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-04 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.88560 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.913, 46.630, 59.787 |
Unit cell angles | 90.00, 107.12, 90.00 |
Refinement procedure
Resolution | 57.140 - 1.199 |
R-factor | 0.18611 |
Rwork | 0.185 |
R-free | 0.20978 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xcj |
RMSD bond length | 0.008 |
RMSD bond angle | 1.220 |
Data reduction software | iMOSFLM (1.0.4) |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.140 | 1.260 |
High resolution limit [Å] | 1.199 | 1.199 |
Rmerge | 0.099 | 0.404 |
Number of reflections | 66281 | |
<I/σ(I)> | 9.6 | 3.1 |
Completeness [%] | 96.3 | 94.3 |
Redundancy | 5.6 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.1M HEPES, PEG 3350 |