4XDX
The crystal structure of soluble human interleukin 8 expressed in Pichia pastoris
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-21 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.806 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 40.093, 40.093, 89.269 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.939 - 0.950 |
| R-factor | 0.1216 |
| Rwork | 0.121 |
| R-free | 0.13560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3il8 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.132 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.000 | 0.970 |
| High resolution limit [Å] | 0.950 | 0.950 |
| Rmerge | 0.050 | 0.743 |
| Number of reflections | 52929 | |
| <I/σ(I)> | 37.81 | 2.3 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 8.7 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 0.17 M Ammonium acetate, 0.085 M Sodium citrate tribasic dihydrate pH 5.6, 20% w/v Polyethylene glycol 4,000, 15% v/v Glycerol |
