4XDM
N-terminal domain of Hsp90 from Dictyostelium discoideum in complex with Geldanamycin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-25 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.95372 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 41.400, 44.630, 60.160 |
Unit cell angles | 90.00, 107.93, 90.00 |
Refinement procedure
Resolution | 57.240 - 1.500 |
R-factor | 0.18376 |
Rwork | 0.182 |
R-free | 0.22426 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4xcj |
RMSD bond length | 0.008 |
RMSD bond angle | 1.215 |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.240 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.048 | 0.682 |
Number of reflections | 33584 | |
<I/σ(I)> | 12.5 | 2.2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 4.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.1 M Hepes, PEG 3350 |