4XDI

Structure of Chlamydomonas reinhardtii THB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-15
Detector	PSI PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	P 61 2 2
Unit cell lengths	144.513, 144.513, 79.913
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.300 - 1.893
R-factor	0.1884
Rwork	0.187
R-free	0.21750
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.118
Data scaling software	XDS
Phasing software	SHELX
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.300	1.961
High resolution limit [Å]	1.893	1.893
Rmerge	0.082
Number of reflections	39522
<I/σ(I)>	13.81	0.96
Completeness [%]	99.9	98.97
Redundancy	10.3	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	298	1.8 M ammonium sulfate, 20% glycerol, 0.1 M glycine
2