4XD8
N-terminal domain of Hsp90 from Dictyostelium discoideum in complex with ANP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.490, 45.750, 59.850 |
| Unit cell angles | 90.00, 107.55, 90.00 |
Refinement procedure
| Resolution | 57.060 - 1.550 |
| R-factor | 0.16303 |
| Rwork | 0.161 |
| R-free | 0.19953 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xcj |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.483 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.060 | 1.630 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.078 | 0.461 |
| Number of reflections | 31081 | |
| <I/σ(I)> | 11.7 | 3.2 |
| Completeness [%] | 99.6 | 99.1 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 0.1M Hepes, PEG3350 |
