4XCX
METHYLTRANSFERASE DOMAIN OF SMALL RNA 2'-O-METHYLTRANSFERASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 166.025, 166.025, 74.478 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.980 - 2.840 |
R-factor | 0.2263 |
Rwork | 0.225 |
R-free | 0.24910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3jwg |
RMSD bond length | 0.008 |
RMSD bond angle | 0.960 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | BALBES |
Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.000 | 35.000 | 2.940 |
High resolution limit [Å] | 2.840 | 6.110 | 2.840 |
Rmerge | 0.098 | 0.039 | 0.731 |
Rmeas | 0.103 | 0.040 | 0.763 |
Rpim | 0.028 | 0.011 | 0.209 |
Total number of observations | 180091 | ||
Number of reflections | 14604 | ||
<I/σ(I)> | 24.25 | 2.08 | |
Completeness [%] | 98.4 | 97 | 95.7 |
Redundancy | 12.3 | 11.5 | 11.7 |
CC(1/2) | 0.999 | 0.882 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.6 | 293 | 2.4 M NACL, 0.1 M TRIS-HCL, PH 8.6,4% 1,3-PROPANEDIOL |