4XCR
Monomeric Human Cu,Zn Superoxide dismutase, loops IV and VII deleted, apo form, mutant I35A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 70.820, 70.820, 70.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.133 - 3.602 |
| R-factor | 0.1906 |
| Rwork | 0.185 |
| R-free | 0.23920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bcz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.780 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP (11.0.05) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.130 | 3.940 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.326 | 0.663 |
| Number of reflections | 2203 | |
| <I/σ(I)> | 2.3 | 1.8 |
| Completeness [%] | 92.8 | 93.8 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5.5 | 293 | 0.1 M Bis Tris and 2.0 M ammonium sulfate |
