4XCP
Fatty Acid and Retinol binding protein Na-FAR-1 from Necator americanus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 4 3 2 |
| Unit cell lengths | 121.123, 121.123, 121.123 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.380 - 2.140 |
| R-factor | 0.2039 |
| Rwork | 0.203 |
| R-free | 0.22230 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | BUSTER (2.10.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.380 | 2.270 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.142 | 0.785 |
| Number of reflections | 17353 | |
| <I/σ(I)> | 25.5 | 4.2 |
| Completeness [%] | 99.9 | 97 |
| Redundancy | 28.9 | 14.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | 38 % PEG 300, 100 mM phosphate citrate pH 4.2 |
