4XCO
Signal-sequence induced conformational changes in the signal recognition particle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.984 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 91.227, 112.971, 121.204 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.025 - 2.900 |
| R-factor | 0.2112 |
| Rwork | 0.209 |
| R-free | 0.25760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ndb |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.521 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.025 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.073 | 0.899 |
| Number of reflections | 28330 | |
| <I/σ(I)> | 13.7 | 1.9 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 6.5 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | The complex (3mg/ml) was mixed 1:1 with 35% 2-Methyl-2,4-pentanediol (MPD), 200 mM NaCl, 80 mM MgCl2, 100 mM sodium acetate |
