4XCO
Signal-sequence induced conformational changes in the signal recognition particle
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-12 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.984 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 91.227, 112.971, 121.204 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.025 - 2.900 |
R-factor | 0.2112 |
Rwork | 0.209 |
R-free | 0.25760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3ndb |
RMSD bond length | 0.008 |
RMSD bond angle | 0.521 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.025 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.073 | 0.899 |
Number of reflections | 28330 | |
<I/σ(I)> | 13.7 | 1.9 |
Completeness [%] | 99.7 | 99.7 |
Redundancy | 6.5 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | The complex (3mg/ml) was mixed 1:1 with 35% 2-Methyl-2,4-pentanediol (MPD), 200 mM NaCl, 80 mM MgCl2, 100 mM sodium acetate |