4XCL

N-terminal domain of Hsp90 from Dictyostelium discoideum in complex with AGS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97625
Spacegroup name	P 1 21 1
Unit cell lengths	41.867, 46.754, 59.487
Unit cell angles	90.00, 106.81, 90.00

Refinement procedure

Resolution	56.940 - 1.210
R-factor	0.16017
Rwork	0.159
R-free	0.18144
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4xcj
RMSD bond length	0.008
RMSD bond angle	1.315
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.940	1.280
High resolution limit [Å]	1.210	1.210
Rmerge	0.060	0.369
Number of reflections	64120
<I/σ(I)>	18.6	5.2
Completeness [%]	95.7	92.5
Redundancy	9.1	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	290	0.1M Hepes, PEG 3350