4XCH

S-ribosylhomocysteinase from Streptococcus suis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	74.890, 86.250, 103.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.177 - 2.200
R-factor	0.1874
Rwork	0.185
R-free	0.23310
RMSD bond length	0.008
RMSD bond angle	1.146
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	38.200
High resolution limit [Å]	1.900
Number of reflections	50447
<I/σ(I)>	6.8
Completeness [%]	99.0
Redundancy	13.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2M potassium-sodium tartrate, 0.1M sodium citrate (pH 6.5), 1M ammonium sulfate