Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9322 |
| Spacegroup name | H 3 |
| Unit cell lengths | 81.610, 81.610, 33.729 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.499 |
| R-factor | 0.2217 |
| Rwork | 0.218 |
| R-free | 0.25370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3BRR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.055 |
| Data scaling software | HKL-2000 (HKL-2000) |
| Phasing software | PHENIX ((phenix.refine: 1.9_1692)) |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.567 |
| High resolution limit [Å] | 1.499 | 1.499 |
| Rmerge | 0.043 | 0.177 |
| Number of reflections | 9224 | |
| <I/σ(I)> | 15.4 | 2.35 |
| Completeness [%] | 99.9 | 83 |
| Redundancy | 4.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.3M MAGNESIUM SULFATE, 0.1M MES |
