4XBR
In cellulo Crystal Structure of PAK4 in complex with Inka
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2013-07-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 63 |
| Unit cell lengths | 144.040, 144.040, 62.510 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.200 - 2.940 |
| R-factor | 0.19113 |
| Rwork | 0.189 |
| R-free | 0.22973 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fie |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.498 |
| Data reduction software | xia2 (0.3.6.0) |
| Data scaling software | xia2 (0.3.6.0) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 44.200 |
| High resolution limit [Å] | 2.940 |
| Number of reflections | 15517 |
| <I/σ(I)> | 10.9 |
| Completeness [%] | 97.3 |
| Redundancy | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | IN CELL | 310.15 | In cellulo growth |
