4XBH

Soluble rabbit neprilysin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Collection date	1999-04-22
Detector	ADSC QUANTUM 210
Wavelength(s)	1.100
Spacegroup name	P 21 21 21
Unit cell lengths	69.361, 108.142, 212.784
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.601 - 2.114
R-factor	0.167
Rwork	0.166
R-free	0.21130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dmt
RMSD bond length	0.003
RMSD bond angle	0.684
Data scaling software	HKL-2000 (1.12.d)
Refinement software	PHENIX (1.9-1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.056	0.046	0.117
Total number of observations	441341
Number of reflections	88988
<I/σ(I)>	16
Completeness [%]	94.4	99.2	63.3
Redundancy	4.96

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	PEG 4000, magnesium chloride, cacodylate