4XB7

Crystal structure of Dscam1 isoform 4.4, N-terminal four Ig domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	100.212, 100.212, 351.761
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.106 - 4.004
R-factor	0.2252
Rwork	0.224
R-free	0.25610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v5m
RMSD bond length	0.004
RMSD bond angle	0.867
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.106
High resolution limit [Å]	4.000
Number of reflections	15793
<I/σ(I)>	7.6
Completeness [%]	99.9
Redundancy	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.1M HEPES pH7.5, 0.5M Magnesium Chloride, 20% (w/v) PEG 3350