4XB4
Structure of the N-terminal domain of OCP binding canthaxanthin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.251, 64.112, 53.634 |
| Unit cell angles | 90.00, 114.89, 90.00 |
Refinement procedure
| Resolution | 38.757 - 1.544 |
| R-factor | 0.1479 |
| Rwork | 0.147 |
| R-free | 0.15990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Truncated version of 3MG1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.042 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1690)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 1.600 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.025 | 0.315 |
| Number of reflections | 46069 | |
| <I/σ(I)> | 13.8 | 2.52 |
| Completeness [%] | 99.5 | 96.33 |
| Redundancy | 7.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 100 mM citric acid BIS-TRIS propane pH 5.5, 24% poly-ethylene glycol 3350 |
