4XAX

Crystal structure of Thermus thermophilus CarD in complex with the Thermus aquaticus RNA polymerase beta1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	93
Detector technology	CCD
Collection date	2012-07-20
Detector	ADSC QUANTUM 315
Wavelength(s)	1.1
Spacegroup name	I 4
Unit cell lengths	149.321, 149.321, 52.256
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.323 - 2.404
R-factor	0.1615
Rwork	0.156
R-free	0.18030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4l5g 3mlq
RMSD bond length	0.010
RMSD bond angle	1.182
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.400	5.170	2.404
Rmerge	0.077	0.034	0.961
Total number of observations	138939
Number of reflections	22705
<I/σ(I)>	9.9
Completeness [%]	100.0	99.6	100
Redundancy	6.1	5.9	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	295	Ammonium sulfate, sodium acetate, ethylene gycol