4XAT

Crystal structure of the olfactomedin domain from noelin/pancortin/olfactomedin-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	80
Detector technology	CCD
Collection date	2014-04-04
Detector	RAYONIX MX300HS
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	39.681, 68.016, 95.659
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.266 - 2.113
R-factor	0.162
Rwork	0.157
R-free	0.20430
RMSD bond length	0.010
RMSD bond angle	1.196
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4 1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	32.270
High resolution limit [Å]	2.110
Number of reflections	15126
<I/σ(I)>	9.4
Completeness [%]	97.9
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	Hepes pH 7.5 PEG 400 PEG 3000 Glycerol