4XAL
Crystal structure of the conserved core domain of VP22 from HSV-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-05-31 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 64.940, 64.940, 107.645 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.923 - 1.869 |
| R-factor | 0.2105 |
| Rwork | 0.208 |
| R-free | 0.25110 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.964 |
| Data reduction software | HKL-2000 |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.940 |
| High resolution limit [Å] | 1.870 | 4.030 | 1.870 |
| Rmerge | 0.052 | 0.029 | 0.289 |
| Total number of observations | 119894 | ||
| Number of reflections | 11585 | ||
| <I/σ(I)> | 16.5 | ||
| Completeness [%] | 98.7 | 95.4 | 99.8 |
| Redundancy | 10.3 | 9.5 | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 40% PEG 300 and 0.1 M Phosphate citrate pH 5 |
