4XAI
Crystal Structure of red flour beetle NR2E1/TLX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-18 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.07820 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.782, 84.098, 262.437 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.270 - 2.600 |
R-factor | 0.2111 |
Rwork | 0.207 |
R-free | 0.26160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID's 4MY2 and 4LOG |
RMSD bond length | 0.004 |
RMSD bond angle | 0.908 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.730 |
High resolution limit [Å] | 2.580 | 2.600 |
Rmerge | 0.078 | |
Number of reflections | 81089 | |
<I/σ(I)> | 7.8 | 1.5 |
Completeness [%] | 92.8 | 85.9 |
Redundancy | 2.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 298 | PEG 3350 |