4XAH

X-ray crystal structure of S. cerevisiae Cgi121

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-01
Detector	MAR CCD 165 mm
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	75.650, 76.900, 84.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.1878
Rwork	0.185
R-free	0.23650
RMSD bond length	0.007
RMSD bond angle	0.999
Data reduction software	XDS
Phasing software	SHELXDE
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.570
High resolution limit [Å]	2.500	2.500
Number of reflections	32377
<I/σ(I)>	14.3
Completeness [%]	99.3	99.3
Redundancy	3.6	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	10 mM Tris-HCl pH 7.5, 100mM NaCl, 10mM 2-mercaptoethanol,0.6 M sodium citrate, 0.05 M sodium citrate pH 5.0